README for Supplementary figure X.csv 
*** This file contains the raw data obtained on DNA, DNA-MJ1647 K80E, DNA-MJ1647 E95K and DNA-MJ1647 K80E E95K complexes using bridging assay experiments represented in Figure 3A of 
Article: DNA-bridging by an archaeal histone variant via a unique tetramerisation interface 
Authors: Ofer, Blombach, Erkelens, Barker, Schwab, Smollett, Matelska, Fouqueau, van der Vis, Kent, Pinotsis, Dame and Werner
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl and f.werner@ucl.ac.uk

Legend Suppelementary figure X: DNA bridging assays with salt bridge mutants of MJ1647 K80E and E95K and the double mutant K80E/E95K compared to wild-type MJ1647. The protein concentration (monomer) is plotted against the recovery of radio-labelled prey DNA.

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of Supplementary figure X. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of MJ1647 K80E/E95K/K80E E95K added to sample in micromolar
Column E: Counts per minutes 
Column F: DNA recovery (%)

Columns B, C and D indicate the contents of the sample. Column E indicates the radioactivity of the sample. 
Column F indicates the DNA recovery, which is calculated by subtracting the value in column E of the control 
(sample minus bait DNA) from the sample itself. This value is then divided by the values in column E of sample 1 
and multiplied by 100%. 









